Ann gave birth to twins on 21 Sep 1857 in Deep River, Middlesex, Connecticut: Charles Lewis and Annie Maria Juchau. Annie was stillborn and was buried the same day in "Free Ground" in the Fountain Hill Cemetery. Charles Lewis lived for over two years before passing away on 20 Sep 1859. He was likewise buried in "Free Ground" of the Fountain Hill Cemetery on the day of his death. The loss of each of these children must have been heartbreaking.
Soon after the loss of Lewis, the family returned to New York City. They are living at 18 Eldredge in the 1860 City Directory of New York. In 1865 they're living at 158 Prince and in 1866 at 153 Prince. We currently do not know what address they were living at on 23 Aug 1862 when Annie Louise was born, but it was very likely one of these two or another address in southern Manhattan.
See the Google Map Juchau Homes and Haunts for a map showing these locations.